Evaluation of Acetyl- and Butyrylcholinesterase Enzyme Inhibitory Activities and Cytotoxic Activities of Anthraquinone Derivatives

In this study, the enzyme activity of anthraquinone compounds which were synthesized beforehand by our research group was investigated. Molecular docking studies performed for 1-(4-aminophenylthio)anthracene-9,10-dione (3) and 1-(4-chlorophenylthio)anthracene-9,10-dione (5). Compound 3 from reaction 1-chloroanthraquinone (1) 4-aminothiophenol (2). 5 4-chlorothiophenol (4). Anthraquinone analogs (3, 5) with a new method made Inhibitory effects investigated against acetylcholinesterase (AChE) butyrylcholinesterase (BuChE) enzymes are related to Alzheimer’s Disease (AD). Compounds exhibited strong anti-acetyl- butyryl-cholinesterase inhibition activities than galanthamine used as standard compound (92.11±1.08 80.95±1.77 %, respectively). The EHOMO-ELUMO values, molecular descriptors, calculated UV-Vis spectra derivatives computed B3LYP/6-31+G(d,p) levels in CHCl3 phase. Based on fluorescence property skeleton, bioactive analogue (5) MTT test determine cytotoxic thioanthraquinone molecules 5. analyses, showed highest effect Ishikawa cells at dose 10 µg/mL, while 50 µg/mL. cell viability 84.18% µg/mL 75.02%